Influence of Spodoptera frugiperda powder supplement on the production and concentration of Metarhizium anisopliae conidia
Keywords:
strains, spores, fall armyworm, entomopathogenic fungiAbstract
Entomopathogenic fungi represent a promising option for pest management; however, fungal pathogenicity tends to decrease over time. Therefore, it is essential to obtain a culture medium that provides the necessary nutrients for their development and maintains virulence. Based on this, the objective of this research was to evaluate the effect of different doses of a powdered supplement made from Spodoptera frugiperda on the spore concentration (mL-1) in strains of the fungus Metarhizium anisopliae. To achieve this, a completely randomized design was established, where four treatments with three replicates were evaluated. Potato Dextrose Agar medium was used as a control (T1). The supplemented Potato Dextrose Agar media were supplemented with S. frugiperda powder at concentrations of T2=0.5%, T3=1.0%, and T4=1.5%. The results show that the highest concentration of M. anisopliae spores was in T3, where 1% S. frugiperda powder supplement was added, with a concentration of 2.8X10⁸ spores - mL-1. Therefore, these findings suggest that this supplement could favor the concentration and mass production of entomopathogenic fungi under laboratory and industrial-scale conditions.
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